High-Performance Dental Resins Containing a Starburst Monomer.

Dimethacrylate-based chemistries feature extensively as resin monomers in dental resin-based materials due to their distinguished overall performance. However, challenges endure, encompassing inadequate mechanical attributes, volumetric shrinkage, and estrogenicity. Herein, we first synthesized a novel resin monomer, 9-armed starburst polyurethane acrylate (NPUA), via the grafting-onto approach. Compared to the primary commercial dental monomer 2,2-bis [p-(2'-hydroxy-3'-methacryloxypropoxy) phenyl] propane (Bis-GMA) (with a viscosity of 1,174 ± 3 Pa·s and volumetric shrinkage of 4.7% ± 0.1%), the NPUA monomer achieves the lower viscosity (158 ± 1 Pa·s), volumetric shrinkage (2.5% ± 0.1%), and cytotoxicity (P < 0.05). The NPUA-based resins exhibit the higher flexural strength, flexural modulus, hardness, and hydrophobicity and lower volumetric shrinkage, water absorption, and solubility compared to the Bis-GMA (70 wt%)/TEGDMA (30 wt%) resins. The NPUA-based composites exhibit significantly higher flexural strength, flexural modulus, and hardness and lower volumetric shrinkage (171.4 ± 3.0 MPa, 12.6 ± 0.5 GPa, 2.0 ± 0.2 GPa, and 3.4% ± 0.2%, respectively) compared to the Bis-GMA group (120.3 ± 4.7 MPa, 9.4 ± 0.7 GPa, 1.5 ± 0.1 GPa, and 4.7% ± 0.2%, respectively; P < 0.05). This work presents a viable avenue for augmenting the physicochemical attributes of dental resins.

Enhanced Bonding to Caries-Affected Dentin Using an Isocyanate-Based Primer.

Dental caries is the most common oral disease and the most common cause of resin restorations. In minimally invasive dentistry, the principle behind cavity preparation is to remove external caries-infected dentin (CID) and preserve internal caries-affected dentin (CAD) and sound dentin (SD). The cavity floor is mainly composed of CAD, but the poor bonding performance of CAD has become a widespread concern. This study evaluated the performance of a new collagen-reactive monomer (ITCM) used as a primer to improve the bonding performance of CAD. The experimental specimens were grouped as follows: SD, CAD, and ITCM-pretreated CAD (CAD-ITCM). Dentin slices were obtained for attenuated total reflectance-Fourier transform infrared (ATR-FTIR) analysis. The bonded samples were subjected to microtensile bond strength analysis after 24 h of water storage or aging by thermocycling, and the bonding interface quality was evaluated by nanoleakage assessment, interfacial nanoindentation testing, and in situ zymography. Cytotoxicity experiments with ITCM were performed. ATR-FTIR showed that the isocyanate groups in ITCM can covalently bind and form hydrogen bonds with the collagen in CAD to mediate chemical bonding. ITCM pretreatment significantly improved the bond strength of CAD (P < 0.05), reduced interfacial nanoleakage, improved the sealing of the bonding interface, enhanced the homogeneity of the hybrid layer, and inhibited matrix metalloproteinase activity. In addition, ITCM presented acceptable biocompatibility for dental restorative application. Taken together, this study reported the application of ITCM to induce collagen-based chemical bonding in the CAD bonding system, which fills the gap in strategies to improve the bonding performance of CAD immediately and after aging and has important clinical application prospects.

Serotonin regulates osteoblast proliferation and function in vitro

The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and mineralization at low concentrations, but this inhibitory effect was mitigated at relatively high concentrations. Six of the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, and 5-HT2C) were found to exist in rat osteoblasts. Of these, 5-HT2A and 5-HT1B receptors had the highest expression levels, at both early and late stages of differentiation. Our results indicated that 5-HT can regulate osteoblast proliferation and function in vitro.

Serotonin regulates osteoblast proliferation and function in vitro.

The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and mineralization at low concentrations, but this inhibitory effect was mitigated at relatively high concentrations. Six of the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, and 5-HT2C) were found to exist in rat osteoblasts. Of these, 5-HT2A and 5-HT1B receptors had the highest expression levels, at both early and late stages of differentiation. Our results indicated that 5-HT can regulate osteoblast proliferation and function in vitro.

Evaluating the clinical significance of serum HE4 levels in lung cancer and pulmonary tuberculosis.

OBJECTIVE: To evaluate whether serum human epididymis protein 4 (HE4) levels could be used as a marker to differentiate lung cancer from pulmonary tuberculosis (PTB) and as a prognostic indicator in patients with non-small-cell lung cancer (NSCLC). DESIGN: HE4 levels were measured in serum samples from 106 healthy controls, 190 lung cancer patients, 114 patients with PTB and 24 patients with pneumonia using enzyme-linked immunosorbent assay. RESULTS: Serum HE4 levels in lung cancer patients were significantly higher than those in patients with PTB and healthy controls (P < 0.001). Using the optimal cut-off value of 94.01 pmol/l, HE4 levels distinguished lung cancer from PTB with a sensitivity of 61.6% and a specificity of 93.0%. After adjusting for age and smoking status, a binary unconditional logistic regression model provided a sensitivity of 67.4% and a specificity of 86.0% for differentiating between these two diseases. In the NSCLC group, serum HE4 levels were significantly higher in patients at advanced stages (Stage I-II vs. Stage III-IV, P = 0.02). Higher levels of serum HE4 (≥ 83.90 pmol/l) were significantly correlated with a worse 3-year survival rate for patients with NSCLC (P < 0.05). CONCLUSION: Serum HE4 may be used as a potential marker to differentiate lung cancer from PTB and healthy controls. In addition, higher levels of HE4 predict poor prognosis in NSCLC patients.

Centrifugal force model for pedestrian dynamics.

In this paper, a centrifugal force model is developed for pedestrian dynamics. The effects of both the headway and the relative velocity among pedestrians are taken into account, which can be expressed by a "centrifugal force" term in dynamic equation. The jamming probability due to the arching at exits for crowd flows is provided. A quantitative analysis of the crowd flowing out of a hall shows that the average leaving time T is a function of the exit width W in negative power. The related simulation indicates that the proposed model is able to reproduce the self-organization phenomena of lane formation for sparse flows.

Stabilization analysis and modified Korteweg-de Vries equation in a cooperative driving system.

Two lattice traffic models are proposed by incorporating a cooperative driving system. The lattice versions of the hydrodynamic model of traffic flow are described by the differential-difference equation and difference-difference equation, respectively. The stability conditions for the two models are obtained using the linear stability theory. The results show that considering more than one site ahead in vehicle motion leads to the stabilization of the system. The modified Korteweg-de Vries equation (the mKdV equation, for short) near the critical point is derived by using the reductive perturbation method to show the traffic jam which is proved to be described by kink-anti-kink soliton solutions obtained from the mKdV equations.

Stabilization effect of traffic flow in an extended car-following model based on an intelligent transportation system application.

An extended car following model is proposed by incorporating an intelligent transportation system in traffic. The stability condition of this model is obtained by using the linear stability theory. The results show that anticipating the behavior of more vehicles ahead leads to the stabilization of traffic systems. The modified Korteweg-de Vries equation (the mKdV equation, for short) near the critical point is derived by applying the reductive perturbation method. The traffic jam could be thus described by the kink-antikink soliton solution for the mKdV equation. From the simulation of space-time evolution of the vehicle headway, it is shown that the traffic jam is suppressed efficiently with taking into account the information about the motion of more vehicles in front, and the analytical result is consonant with the simulation one.

The adsorption behaviour of dimethyltin from seawater onto sedimentary phase in Tianjin Harbor.

The adsorption of dimethyltin from a seawater matrix onto suspended particulate matter was studied. The influences of pH and salinity, adsorption isotherms at various temperatures, and the adsorption rate under certain experimental conditions were determined. It was found that the adsorption capacity was highest at c. pH 6 and decreased with increasing salinity. The adsorption behaviour at various temperatures was described best by a Langmuir isotherm. The adsorption capacity of dimethyltin was higher in natural seawater than in artificial seawater at the same temperature.